Fermenting — in Space?

Microbes that coexist on plants influence a crop’s size, shape, color, flavor and yield. What would these microbes do in microgravity? The International Space Station (ISS) is testing to find these results. 

Flight engineer Shannon Walker (pictured) shows sample bags collected for the Grape Juice Fermentation in Microgravity Aboard ISS study. Astronauts will observe the fermentation process, measuring microbial differences. Back on earth, a matching, control sample is being observed in an environmental control chamber that mimics the ISS ambient temperature. The space and earth samples then will be analyzed for changes. Both flight and ground control samples are analyzed post-investigation for genetic change.

Read more (NASA)

A new study on kefir found that the individual dominant species of Lactobacillus bacteria in kefir grains cannot survive in milk on their own. The bacteria need one another to create the fermented dairy drink, “feeding on each other’s metabolites in the kefir culture.”

The research, conducted by EMBL (Europe’s laboratory for life and sciences) and Cambridge University’s Patil group and published in Nature Microbiology, illustrates a dynamic of microbes that had eluded scientists. Though scientists knew microorganisms live in communities and depend on each other to survive, “mechanistic knowledge of this phenomenon has been quite limited,” according to a press release on the research.

“Cooperation allows them to do something they couldn’t do alone,” says Kiran Patil, group leader and author of the paper. “It is particularly fascinating how L. kefiranofaciens, which dominates the kefir community, uses kefir grains to bind together all other microbes that it needs to survive — much like the ruling ring of the Lord of the Rings. One grain to bind them all.”

To make kefir, it takes a team. A team of microbes.

The group studied 15 samples of kefir, one of the world’s oldest fermented food products. Below are highlights from a press release on the published results. 

A Model of Microbial Interaction

Kefir first  became popular centuries ago in Eastern Europe, Israel, and areas in and around Russia. It is composed of ‘grains’ that look like small pieces of cauliflower and have fermented in milk to produce a probiotic drink composed of bacteria and yeasts.

People were storing milk in sheepskins and noticed these grains that emerged kept their milk from spoiling, so they could store it longer,” says Sonja Blasche, a postdoc in the Patil group and an author of the paper. Because milk spoils fairly easily, finding a way to store it longer was of huge value.”

To make kefir, you need kefir grains, which must come from another batch of kefir. They cannot be made artificially. The grains are added to milk, to ferment and grow. Approximately 24 to 48 hours later (or, in the case of this research, 90 hours later), the kefir grains have consumed the available nutrients. The grains have grown in size and number, and are removed and added to fresh milk — to begin the process anew.

For scientists,  kefir is more than just a healthy beverage: it’s an easy-to-culture model microbial community for studying metabolic interactions. And while kefir is quite similar to yogurt in many ways – both are fermented or cultured dairy products full of ‘probiotics’ – kefir’s microbial community is far larger, including not just bacterial cultures but also yeast.

A “Goldilocks Zone”

While scientists know that microorganisms often live in communities and depend on their fellow community members for survival, mechanistic knowledge of this phenomenon has been quite limited. Laboratory models historically have been limited to two or three microbial species, so kefir offers – as Patil describes – a ‘Goldilocks zone’ of complexity that is not too small (around 40 species), yet not too unwieldy to study in detail.

Blasche started this research by gathering kefir samples from several sources. Though most  were obtained in Germany, they may have  originated elsewhere, grown from kefir grains  passed down through the years..

“Our first step was to look at how the samples grow. Kefir microbial communities have many member species with individual growth patterns that adapt to their current environment. This means fast- and slow-growing species and some that alter their speed according to nutrient availability, Blasche  says. This is not unique to the kefir community. However, the kefir community had a lot of lead time for coevolution to bring it to perfection, as they have stuck together for a long time already.”

Cooperation is key

Finding out the extent and nature of  cooperation among kefir microbes was far from straightforward. Researchers combined a variety of state-of-the-art methods, such as metabolomics (studying metabolites’ chemical processes), transcriptomics (studying the genome-produced RNA transcripts) and mathematical modelling. These processes  revealed not only key molecular interaction agents like amino acids, but also the contrasting species dynamics between  grains and  milk.

“The kefir grain acts as a base camp for the kefir community, from which community members colonise the milk in a complex yet organised and cooperative manner,” Patil says. “We see this phenomenon in kefir, and then we see it’s not limited to kefir. If you look at the whole world of microbiomes, cooperation is also a key to their structure and function.”

In fact, in another paper from Patil’s group (in collaboration with EMBL’s Bork group) in Nature Ecology and Evolution, scientists combined data from thousands of microbial communities across the globe – from in soil to in the human gut – to understand similar cooperative relationships. In this second paper, the researchers used advanced metabolic modelling to show that the co-occurring groups of bacteria, groups that are frequently found together in different habitats, are either highly competitive or highly cooperative. This stark polarization hadn’t been observed before, and sheds light on evolutionary processes that shape microbial ecosystems. While both competitive and cooperative communities are prevalent, the cooperators seem to be more successful in terms of higher abundance and occupying diverse habitats — stronger together!

Fermenti Foods has teamed up with a local salt producer to help food insecurity in their local county of Madison County, North Carolina.

Meg Chamberlain (pictured) donates jars of her fermented kraut and carrots to the Beacon of Hope Food Bank. She’s also helped the food bank preserve fresh food donations. And now she’s adding recipes for sauerkraut and tutorials on YouTube. Chamberlain says fermentation is a useful tool for home preservation.

“In addition to the fact that more people are growing, they need to know what to do with what they’re growing in their gardens,” she said.

Read more (Citizen Times)

Chocolate’s Beer Roots

Chocolate beer sounds like a faddish flavor, but its roots run deep. Pre-Columbian Mesoamericans fermented cacao fruit to make a beer-like drink called chicha.

Chicha is usually made from corn, but there are regional variations made from other new-world plants like potatoes, peanuts and cacao beans,” reads an article from The Seattle Times. Chicha was studied by archaeology professors at Cornell and Berkeley who found “The roots of the modern chocolate industry can be traced back to this primitive fermented drink.”

Read more (The Seattle Times)

Do probiotic supplements deliver the same benefits as fermented foods? Dr. Gail Cresci says to be leery of prebiotic and probiotic pills.

“There are challenges to keeping microbes viable in encapsulated tablets,” says Cresci, director of Nutrition Research for the Cleveland Clinic’s Department of Pediatric Gastroenterology, Hepatology and Nutrition. “It’s also very, very important to know that each strain of bacteria is not the same as the next. For example, lactobacillus has hundreds of different strains, and each one may behave differently. People like to use supplements because they like to think one size fits all, but it doesn’t.”

“Take in prebiotics and probiotics through food sources. Yogurt with added probiotic bacterial strains is much better to consume than supplements also because as it’s been waiting for you to eat it, it’s been producing more beneficial metabolites. When you eat it, you get all that.”

Read more (American Heart Association/U.S. News & World Report)

After Dr. Bob Hutkins finished a presentation on fermented foods during a respected nutrition conference, the first audience question was from someone with a PhD in nutrition: “What are fermented foods?”

“I thought ‘Doesn’t everyone know what fermentation is?’ I realized, we do need a definition. Those of us that work in this field know what we’re talking about when we say fermented foods, but even people trained in foods do not understand this concept,” says Hutkins, a professor of food science at the University of Nebraska-Lincoln. He presented The New Definition of Fermented Foods during a webinar with TFA

Hutkins was part of a 13-member interdisciplinary panel of scientists that released a consensus definition on fermented foods. Their research, published this month in Nature Reviews Gastroenterology & Hepatology, defines fermented foods as: “foods made through desired microbial growth and enzymatic conversions of food components.”

“We needed a definition that conveyed this simple message of a raw food turning into a fermented food via microorganisms,” Hutkins says. “It brings some clarity to many of these issues that, frankly, people are confused about.”

David Ehreth, president and founder of Alexander Valley Gourmet, parent company of Sonoma Brinery (and a TFA Advisory Board member), agreed that an expert definition was necessary.

“As a producer, and having started this effort to put live culture products on the standard grocery shelf, I started doing it as a result of unique flavors that I could achieve through fermentation that weren’t present in acidified products,” Ehreth says. “Since many of us put this on our labels, we should be paying close attention to what these folks are doing, since they are the scientific backbone of our industry.”

Hutkins calls fermented foods “the original shelf-stable foods.” They’ve been used by humankind for over thousands of years, but have mushroomed in popularity in the last 15. Fermented foods check many boxes for hot food trends: artisanal, local, organic, natural, healthy, flavorful, sustainable, innovative, hip, funky, chic, cool and Instagram-worthy.

Nutrition, Hutkins hypothesizes, is a big driver of the public’s interest in fermentation. He noted that Today’s Dietitian has voted fermented foods a top superfood for the past four years. 

Evidence to make bold claims about the health benefits of fermentation, though, is lacking. Hutkins says there is observational and epidemiological evidence. But randomized, human clinical trials — “the highest evidence one can rely on” — are few and small-scale for fermented foods. 

Hutkins shared some research results. One study found that Korean elders who regularly consume kimchi harbor lactic acid bacteria (LAB) in their GI tract, providing compelling evidence that LAB survives digestion and reaches the gut. Another study of cultured dairy products, cheese, fermented vegetables, Asian fermented products and fermented drinks found that most contain over 10 million LAB per gram. 

Still, the lack of credible studies is “a barrier we have to get past,” Hutkins says. There are confirmed health benefits with yogurt and kefir, but this research was funded by the dairy industry, a large trade group with significant resources. 

“I think there’s enough evidence — most of it through these associated studies — to warrant this statement: fermented foods, including those that contain live microorganisms, should be included as part of a healthy diet.”

Probiotics and fermented foods are not equivalent, says Mary Ellen Sanders, PhD and executive science officer of the International Scientific Association of Probiotics & Prebiotics (ISAPP). She advises fermented food producers that don’t meet the criteria of a probiotic to use descriptors such as “live active cultures” or “fermented food with live microbes” on their labels rather than “probiotic.”

“There are quite a few differences between probiotics and many fermented foods. You cannot assume a fermented food is a probiotic food even if it has live cultures present,” says Sanders. She highlighted her 30 years worth of insight into the field during a TFA webinar, Are Fermented Foods Probiotics? 

Some fermented foods do meet these criteria, such as some yogurts and cultured milks that are well-studied. But many traditional fermented foods do not. 

Using multiple peer-reviewed scientific studies and conclusion from expert panels in the fields of probiotics and fermented foods, Sanders shared the ways in which fermented foods and probiotics differ:

  1. Health benefits

By definition, a probiotic must have a documented health benefit. Many fermented foods have not been tested for a health benefit. 

“If you are interested in recommending health benefits from a fermented food in an evidence-based manner, many traditional fermented foods fall short. They don’t have the controlled randomized trials that will provide a causal link between the food and the health benefit,” she says. “A food may be nutritious, but probiotic benefits must stem from the live microbe, not the nutritional composition of the food. Otherwise you just have a nutritious food that happens to have live microorganisms in it. You don’t have a probiotic food.”

  1. Quality studies

In her presentation, Sanders shared  multiple randomized clinical trials on human subjects with supported health evidence for probiotics. But there are few randomized, controlled studies on fermented foods. Most are cohort studies, which inherently have a higher risk of bias and cannot provide a causal link between consuming fermented foods and a health benefit. 

“A strong hypothesis is not the same as proof,” Sanders says. “Evidence for probiotics must meet a higher standard than small associative studies, many of which are tracking biomarkers and not health endpoints.” 

She noted, though, there are some studies on fermented milk and yogurt that show a conferred health benefit.

  1. Strain designation

Though many fermented foods do have live microbes, a probiotic is required to be identified to the strain level. The genus and species should also be properly named according to current nomenclature. Many fermented foods contain undefined microbial composition. Without that strain designation, one can’t tie the scientific evidence on that strain to the probiotic product.

  1. Microbe quantity

Another key differentiator is that probiotics must be delivered at a known quantity that matches the amount that results in a health benefit. Probiotics are typically quantified in colony forming units (or CFUs). 

“A probiotic has a known effective dose. But fermented foods often contain unknown levels of microbes, especially at time of consumption,” Sanders says.

What Can Brands Do?

If food brands keep using the word probiotics as a catch-all to describe a fermented product, the term will lose its utility. Using “probiotics” on food with unsubstantiated proof of probiotics is a misuse of the term.

“When I see a fermented food that says probiotics on it, I very often think what they’re trying to communicate on that label [is that it] contains live microbes,” Sanders says, “because I’m doubting, at least some of the products I see, that they have any evidence of a health benefit. And so they’re just looking for a catchy, single word that will communicate to people that this has live microbes in it. ‘Live active cultures’ is something that resonates with people as well. So why not use that?”

Sanders encourages fermented brands to standardize the terms “live active cultures,” “live microbes,” “live microorganisms” or “fermented food with live microbes.” For products pasteurized after fermentation, there’s a term for them too: “Made with live cultures.”

Controlled human studies on fermented foods can be challenging, Sanders admits. Such studies can be difficult to properly blind, since placebos for foods are hard to design. The fermentation process affects the product taste so that study subjects may know what they are consuming. But the health benefits of fermented foods could be studied, though. She also advises producers to focus on the nutritional value of their food. 

“That’s one thing that really has me excited about this concept of core benefits,” says Maria Marco, PhD, professor  of food science and technology at University of California, Davis (and a member of TFA’s Advisory Board) and moderator of the webinar. “I think it kind of opens the doors to the possibility of fermented fruits and vegetables where there’s certain organisms, microorganisms that we’d expect to be there but again we need to know really if those microorganisms are needed to make those foods healthy.”

By August, any manufacturer labeling their fermented or hydrolyzed foods or ingredients “gluten-free” must prove that they contain no gluten, have never been through a process to remove gluten, all gluten cross-contact has been eliminated and there are measures in place to prevent gluten contamination in production.

The FDA list includes these foods: cheese, yogurt, vinegar, sauerkraut, pickles, green olives, beers, wine and hydrolyzed plant proteins. This category would also include food derived from fermented or hydrolyzed ingredients, such as chocolate made from fermented cocoa beans or a snack using olives.

Read more (JD Supra Legal News

Why do we treat cheese like a guilty pleasure when studies have shown time and again that cheese can be good for you? An article in Wired sheds light on how cheese gained its designation as a villain in the food pyramid. One key element is how many Americans ate in the ‘80s and ‘90s, layering pizza and pasta with heavily-processed cheese.

The article reads: “In the case of cheese, there could be several reasons for the surprising lack of impact on weight, though more research is needed. Cheese is fermented, meaning it has live bacterial cultures. That could have a positive effect on the gut microbiome, which appears to play a role in weight regulation. The fermentation process also creates vitamin K2, or menaquinone, which experimental studies have linked to improved insulin function. Insulin regulates blood sugar levels, hunger, calorie expenditure, and fat storage. (One upshot is that hard, aged cheeses, which are more fermented, probably provide more benefit than soft, less fermented ones.) There’s also some promising research about the benefits of a compound called the milk-fat globule membrane, which is intact in cheese but not in milk or butter.”

Read more (Wired)

A new project is aiming to provide accurate information and resources to the public around the microbial mysteries of fermented foods. EATLAC is a University of California, Davis, project putting scientific knowledge and research behind fermentation.

“A good understanding of food and beverage fermentation is particularly important for people making fermented foods at home so that the foods are made properly and minimize the risk of foodborne illness,” says Maria Marco, PhD, microbiologist and professor in the department of food science and technology at the university (and member of TFA’s Advisory Board). “Access to accurate information about fermented foods is quite important for understanding their roles in healthy diets and what properties about them are different from the starting ingredients.” 

EATLAC officially launched in 2019, with the help of funding from California Department of Food & Agriculture. Led by project directors Marco and Erin DiCaprio, PhD, a food safety expert and extension specialist at UC Davis, the EATLAC team is composed of grad and undergrad students helping to develop science-based guidance on fermented vegetables and fruits. Resources being developed in the project include recipes, consumer surveys, and webinars. This information will be available to the general public, from home fermenters to commercial producers.

The name EATLAC stands for Evaluating And Testing Lacto-ferments Across the Country, but LAC is also a play on words for lactic acid bacteria, the beneficial bacteria present in fermented foods. 

Project objectives include:

  • Developing educational materials on fermented foods.
  • Conducting public education workshops on fermented foods.
  • Measuring the bioactive properties of unpasteurized (fresh) fermented fruits and vegetables produced from specialty crops in California.
  • Disseminating research findings to food processors.

“It is very important to spread reliable information and vetted recipes so that everyone can create something that is great but, more importantly, safe,” says Zoe Mitchell, undergraduate student at UC Davis. “Fermentation requires that food be left out on the counter (in the microbial temperature danger zone) for days on end. To create a safe fermentation environment that supports the growth of the microbes we want, recipe ingredients include things like acid and salt to inhibit pathogens. However, because just a little too much or too little of these ingredients can grow the wrong and potentially dangerous microorganisms, it is paramount to have reliable sources.”

An important role of the student team, Marco says, is to “perform laboratory research to study the microbiota and bioactive properties in fermented fruits and vegetables.” Students will also be responsible for developing informational materials on fermented fruits and vegetables and run the social media accounts. 

Natália Ribeiro, doctoral student at UC Davis, has developed aspects of the project communication. She manages the EATLAC Instagram account, coordinates the posts on FaceBook, and is helping create fermentation fact sheets that will be available on the website. Riberio says she decided to join the project because she wanted to study further the health benefits of fermentation.

“There’s so much more  to know about fermented foods and map out. We want to spread that information in a more validated way,” Riberio says. “People associate fermented foods with flavor, but in a society where we see so many people dealing with diseases, making fermented foods part of your habits could actually alleviate or improve your health in a very easy and even tasty way. Educating people about what is known (and not) is important.”

Since most of the team is not allowed to be in the laboratory due to the pandemic, they are collecting home ferments for analysis. EATLAC is calling on home fermenters around the country to send in their fresh fruit or vegetable ferments. Fresh ferments are samples taken right after fermentation, before refrigeration. The research group will then study the nutritional content and beneficial bacteria. Mitchell adds this allows the team “to create an extensive database on the microbial ecology of various fermentations.” Elements like ingredients, location, salt percentage and vessel type are all tracked to see how these factors affect fermentation. 

The team is currently studying samples from home during the COVID-19 pandemic, but they’re anxious to return to the lab. 

“There is a great and long history of food fermentation research at UC Davis,” Marco notes. The team will update existing informational resources and write new ones within EATLAC. 

For more information about the educational resources, visit EATLAC webinar series.